Fig. 1: The activation of cGAS entails a disorder-to-order transition.
From: The structural basis for 2′−5′/3′−5′-cGAMP synthesis by cGAS
A Cartoon describing the activation of cGAS. B, C An overlay of dsDNA-free and dsDNA-bound wild-type (WT)-mcGAScat structures at the active site (B) and the activation loop (C). PDB ID: 8shu (yellow) is from this work. The PDB ID for each structure is appended in parenthesis hereafter and Supplementary Tables 1–9 list all data collection and refinement statistics. D An overlay of dsDNA-free and dsDNA-bound WT-hcGAScat structures. The “activation loop” in the new structure from this work (PDB ID: 8shz, yellow) is not modeled due to poor electron density. E dsDNA-free WT-hcGAScat bound to cGAMP. The 2Fo-Fc map is contoured at 1.5 σ (same for all 2Fo-Fc maps shown hereafter). The Fo-Fc (omit) maps for all NTPs are shown in Fig. S8. F dsDNA-free WT-hcGAScat bound to pppGpG. The triphosphate was modeled in the absence of electron densities for presentation. G dsDNA-free WT-mcGAScat bound to ATP. The ribose was modeled without density for presentation.
