Fig. 5: TRA-1 and MAB-3 control INS-39 dimorphic neuronal expression.
From: Sex-specific developmental gene expression atlas unveils dimorphic gene networks in C. elegans
a Representative confocal micrographs of the ins-39(syb4915) reporter expression in a wild-type YA hermaphrodite and pan-neuronally masculinized YA hermaphrodites, expressing rab-3p::fem-3. Co-injection marker is indicated by a yellow asterisk. Scale bars represent 10 µm. b Quantification of the number of ins-39(syb4915) GFP-expressing neurons from wild-type, pan-neuronally masculinized, mab-3 (e1240) and pan-neuronally masculinized together with mab-3 (e1240) YA hermaphrodite. Wild-type: n = 15 worms, rab-3p::fem-3: n = 15 worms, mab-3 (e1240): n = 15 worms, rab-3p::fem-3; mab-3 (e1240): n = 14 worms. c Representative confocal micrographs of the ins-39(syb4915) reporter expression in a L1 hermaphrodite mab-3(e1240), YA hermaphrodite mab-3(e1240) and pan-neuronally masculinized together with mab-3 (e1240) YA hermaphrodite. Scale bars represent 10 µm. d Quantification of the number of ins-39(syb4915) GFP-expressing neurons from wild-type L1 hermaphrodite: n = 15 worms and mab-3(e1240) L1 hermaphrodite: n = 14 worms. e A model depicting the molecular elements mediating INS-39 expression in hermaphrodite at L1 and YA stage. f Representative confocal micrographs of the ins-39(syb4915) reporter expression in a wild-type male and pan-neuronally feminized male, expressing rab-3p::tra-2(IC). Co-injection marker is indicated by a yellow asterisk. Scale bars represent 10 µm. g Quantification of the number of ins-39(syb4915) GFP-expressing neurons in wild-type: n = 14 worms, pan-neuronally feminized: n = 15 worms, mab-3 (e1240): n = 14 worms and pan-neuronally feminized together with mab-3 (e1240): n = 14 worms YA male. h Representative confocal micrographs of the ins-39(syb4915) reporter expression in L1 male mab-3(e1240), YA male mab-3(e1240) and pan-neuronally feminized together with mab-3 (e1240) YA male. Scale bars represent 10 µm. i Quantification of the number of ins-39(syb4915) GFP-expressing neurons from wild-type L1 male and mab-3(e1240) L1 male. n = 15 worms per group. j A model depicting the molecular elements mediating INS-39 expression in males at L1 and YA stages. In (b, d, g, i) the box-and-whiskers graph, the center line in the box denotes the median, while the box contains the 25th to 75th percentiles of the dataset, whiskers define the minimum and maximum value with dots showing all points. We performed a two-sided Mann-Whitney test for each comparison. Source data are provided as a Source Data file.
