Fig. 6: Functional characterization of the role of INS-39.
From: Sex-specific developmental gene expression atlas unveils dimorphic gene networks in C. elegans
a Representative confocal micrographs of the ins-39(syb4915) reporter expression in worms grown at 22oC and then transferred to 25 oC or 15 oC for 6 hours. Scale bars represent 10 µm. b Quantification of mean GFP intensity from a in AFD in hermaphrodites and males. hermaphrodites 15 oC: n = 11 worms, males 15 oC: n = 12 worms, hermaphrodites 22 oC: n = 12 worms, males 22 oC: n = 12 worms, hermaphrodites 25 oC: n = 12 worms, males 25 oC: n = 12 worms. a.u, arbitrary units. c Quantification of mean GFP intensity from (a) in ins-39(syb4915) GFP-expressing neurons in males. males 15 oC: n = 16 worms, males 22 oC: n = 14 worms, males 25 oC: n = 14 worms. a.u, arbitrary units. d Schematic of the survival assay on 6 mM tBuOOH and cumulative survival graph on 6 mM tBuOOH of wild-type and ins-39 KO (ety9) hermaphrodites and males. n = 40 worms per group. To assess and compare survival rates, a two-sided Kaplan-Meier method was employed. Statistical significance was determined using the Log-rank (Mantel-Cox) test in Prism (GraphPad). FUDR, 5-fluoro-2′-deoxyuridine. tBuOOH, tert-butyl hydroperoxide. Created with Biorender.com. e Quantification of dauers percent in wild-type and ins-39 KO (ety9) strain in hermaphrodites and males habituated at 27 oC (see methods). Wild-type hermaphrodites: n = 13, ins-39 KO (ety9) hermaphrodites: n = 13, Wild-type males: n = 10, ins-39 KO (ety9) males: n = 11 biological repeats. f Cumulative L1 survival graph of wild-type and INS-39 KO (ety9) hermaphrodites and males. Graphs show the composite of three independent experiments. The n for each group in the final datasets was as follows: Wild-type L1 Herm n = 2676, INS-39 KO (ety9) L1 Herm n = 1421, Wild-type L1 Male n = 2241, INS-39 KO (ety9) L1 Male n = 5453. To assess and compare survival rates, a two-sided Kaplan-Meier method was employed. Statistical significance was determined using the Log-rank (Mantel-Cox) test in Prism (GraphPad). In (b, c, e) the box-and-whiskers graph, the center line in the box denotes the median, while the box contains the 25th to 75th percentiles of the dataset, whiskers define the minimum and maximum value with dots showing all points. We performed (in b, c, e) a two-sided Mann-Whitney test for each comparison. Source data are provided as a Source Data file.
