Fig. 6: PC dendritic calcium responses to tactile stimuli exhibit a linear stimulus-intensity dependence. | Nature Communications

Fig. 6: PC dendritic calcium responses to tactile stimuli exhibit a linear stimulus-intensity dependence.

From: Intrinsic and synaptic determinants of receptive field plasticity in Purkinje cells of the mouse cerebellum

Fig. 6

a, b AAV vectors were injected into Crus I of cerebellar cortex to express Cre-dependent GCaMP6f specifically in PCs. Cutaneous airpuff stimuli were applied to the finger or wrist of the forelimb while calcium responses in PC dendrites were recorded in ipsilateral Crus I under the two-photon microscope. Trials with movement of the limb or body were discarded. c Example field of view with a subset of manually drawn dendritic ROIs and corresponding deconvolved calcium traces below from one 20 s trial with an airpuff delivered to the wrist at 10 ms. Calcium images were obtained with a 62 Hz frame rate, 3–4× digital zoom, and dimensions of 380–669 μm width and 153–270 μm height. Average calcium signals ± SEM for all trials (d) and responsive trials (e). f Raster plots depict trials with both spontaneous and evoked responses to airpuff stimuli with varying pressures (4, 6, 8, and 10 psi). To estimate a spontaneous event rate, 200 ms time windows were randomly selected from the period before the stimulus and aligned to compare with evoked trials that are aligned by stimulus onset. The light orange area represents a 200 ms shuffling window for spontaneous events or a response window (0–200 ms) for evoked events. Trials with detected events within the response window are categorized as responsive trials (blue), whereas those without are considered non-responsive trials (gray). Trials are sorted based on the timing of the first event following the initiation of either time window. g Probability (mean ± SEM) of detecting a calcium event within the defined time windows for spontaneous or responsive conditions. (One-way ANOVA: F[4, 1225] = 45.14, p < 0.001, n = 1230). h Maximum values (mean ± SEM) within the 200 ms shuffled time window or after the initiation of stimulus for evoked calcium transients shown in (d). (One-way ANOVA: F[4, 1225] = 20.62, p < 0.001, n = 1230). GCs granule cells. MFs mossy fibers. PN pontine nuclei. SP spontaneous calcium events. Sh shuffled traces. (246 cells from 18 mice for all analyses).

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