Fig. 7: Physiological tactile-RF plasticity on PC dendrites requires both synaptic and intrinsic plasticity.
a Schematic illustration of the experimental protocol for wrist airpuff tetanization. The recording began with a 20 min baseline recording consisting of 10–12 trials, followed by a wrist airpuff tetanus to potentiate the calcium response. The recording concluded with another 20 min early and late post-tetanus recordings, each consisting of 10–12 trials. Post-tetanus trials began immediately (<2 min) after cessation of the tetanus. b Normalized average calcium signals ± SEM across trials from different genotypes. c Normalized average calcium signals ± SEM across trials of the control condition in WTs where no tetanization protocol was applied. d Normalized average calcium signals ± SEM across trials from control conditions across genotypes where airpuff tetanization was applied to the finger and thus was unmatched to the test location (wrist). The signals shown in (b–d) are normalized by the average amplitude of pre-tetanus calcium transients (y-axis scale denotes normalized value units). Only trials with detected events within the response window (0–200 ms) were included. e Mean ± SEM of the normalized calcium-event amplitudes, calculated as the maximum value within the 0–200 ms time window of individual trials. The data were normalized by the pre-tetanus amplitude. (Two-way repeated measure ANOVA: Fstim[2, 1144] = 0.068, p = 0.935; Fstim x gene[24, 1144] = 4.126, p < 0.001, n = 2073). f Mean ± SEM of the normalized probability of detecting a calcium event within the 0–200 ms time windows. The data were normalized by the pre-tetanus probability. (Two-way repeated ANOVA: Fstim[2, 1356] = 0.520, p = 0.595; Fstim x gene[24, 1356] = 5.274, p < 0.001, n = 2067). Asterisks denote the significance levels of post hoc comparisons using Tukey’s HSD (*p < 0.05; **p < 0.01; ***p < 0.001).
