Fig. 5: MFAP4 and MFAP4C34S interactions with fibroblasts and visualisation of MFAP4-fibrillin-1 complexes. | Nature Communications

Fig. 5: MFAP4 and MFAP4C34S interactions with fibroblasts and visualisation of MFAP4-fibrillin-1 complexes.

From: Microfibril-associated glycoprotein 4 forms octamers that mediate interactions with elastogenic proteins and cells

Fig. 5

A Cell attachment of human dermal fibroblasts to MFAP4, MFAP4C34S, rFBN1-N, and plasma fibronectin (pFN) as control. The proteins were either coated alone as indicated or were first mixed (MFAP4/MFAP4C34S + rFBN1-N) and then coated, or MFAP4/MFAP4C34S were first coated followed by incubation with rFBN1-N (MFAP4/MFAP4C34S » rFBN1-N). Data points represent mean values derived from n = 3 independent experiments, each measured with six technical replicates. Error bars represent the standard error of the mean. Statistical analysis was performed using the two-sided unpaired t-test. p-values are indicated for each comparison. Source data are provided as a Source Data file. B AFM visualisation of the recombinant N-terminal half of fibrillin-1 (rFBN1-N; top panel) or full-length recombinant fibrillin-1 (rFBN1; bottom panel) either alone (left panels) or mixed with MFAP4 (right panels). Some rFBN1-N and rFBN1 molecules are marked by white arrowheads, and some MFAP4 molecules are labelled with black arrowheads. The distance scale bar represents 100 nm for all images; the height scale bar represents 0-1.5 nm for all images.

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