Fig. 5: Anaphase Rif1 modulates NE disassembly.

a Five-fold serial dilutions of log phase cells were plated on minimal PMG media (without thiamine to allow induction of nmt41-gfp-rif1+, integrated at the endogenous rif1+ locus) with or without BA and incubated at 32 °C (3 days) or 25 °C (7 days). b Five-fold serial dilutions of log phase cells at 32 °C were stamped on rich media (YES) containing various concentrations of BA and incubated at 32 °C (3 days) or 19 °C (10 days). c Quantitation of % of dead cells grown in media without or with 0.025% BA at 19 °C for 3 days. Each data point represents one experiment in which >1000 cells were scored. The mean value from three independent experiments is represented for each condition. Exact p values derived from two tailed unpaired T test are represented. d Working model for Rif1’s control of anaphase midregion NE breakdown. Rif1 bound telomere entanglements (top) are shown stretched across the anaphase midregion (middle panel). The lowest panel shows Rif1 coordinating the presence of telomere entanglements with modifications to NE components including Nup60. The arrows point to the different fates of entanglements, determined by the presence or absence of Rif1. Rif1 induces a delay in exposure of entanglements to the cytoplasm, hampering resolution; conversely, timely cytoplasmic exposure, afforded by the absence of Rif1, promotes resolution.