Fig. 5: Application of CuNiP for the selective labeling of various intact proteins (6.5-79.5 kDa) using 1a. | Nature Communications

Fig. 5: Application of CuNiP for the selective labeling of various intact proteins (6.5-79.5 kDa) using 1a.

From: Copper(I)-nitrene platform for chemoproteomic profiling of methionine

Fig. 5

The % conversion to modified proteins was analyzed by MS and the site of modification is determined by MS/MS analysis. Ubiquitin reaction was performed in MeCN:0.01 M citrate buffer (1:4) as a solvent. For denatured lysozyme egg-white, lysozyme human, and α-chymotrpsinogen A, reactions were performed in MeCN:H2O (3:1) as solvent. Figure 5, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license” (Agreement number: IL26NYB6ZR).

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