Fig. 6: Application of CuNiP for selective functionalization of myoglobin and bioactive peptides by affinity tags. | Nature Communications

Fig. 6: Application of CuNiP for selective functionalization of myoglobin and bioactive peptides by affinity tags.

From: Copper(I)-nitrene platform for chemoproteomic profiling of methionine

Fig. 6

a Installation of various affinity tags on myoglobin by reaction with chloramine-T affinity probes, 1h-1j to generate sulfonyl sulfimide conjugates 2h-2j. b MS/MS analysis of 1h-modified myoglobin, 2h showed modification at M131. c Circular Dichroism (CD) analysis of 1a-modified myoglobin showed that the tertiary structure remained intact as compared to unmodified myoglobin. d Substrate scope of CuNiP in selective modification of methionine in bioactive peptides with an affinity tag 1i-probe. Figure 6, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license” (Agreement number: HX26NYAPQ4).

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