Fig. 7: Labeling of native proteins and cell lysate by fluorophore using CuNiP. | Nature Communications

Fig. 7: Labeling of native proteins and cell lysate by fluorophore using CuNiP.

From: Copper(I)-nitrene platform for chemoproteomic profiling of methionine

Fig. 7

a labeling of proteins with alkyne probe 1i using CuNiP followed by click reaction with azide fluorophore and analysis by in-gel fluorescence. b Dose-dependent labeling of a complex cell lysate with 1i using CuNiP followed by click reaction with azide fluorophore as analyzed by in-gel fluorescence. This experiment was repeated (n = 2 biological replicates) with similar results. CB coomassie blue, Fl fluorescence gel Source data are provided as a Source Data file. Figure 7, created with BioRender.com, released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license” (Agreement number: ZB26NYAH42).

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