Fig. 1: Shank3-cKO in the vCA1 using the AAV-mediated CRISPR/Cas9 system impairs social memory. | Nature Communications

Fig. 1: Shank3-cKO in the vCA1 using the AAV-mediated CRISPR/Cas9 system impairs social memory.

From: Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory in mice

Fig. 1

a Schematic illustration of the Shank3-targeting AAV vector. b, c Schematic illustration of the social discrimination test chamber (b) and schedule (c). d Schematic illustration of AAV microinjection. e Representative confocal microscopy images of AAV injected vHPC stained with anti-RFP (red, for mCherry) and DAPI (blue). Scale bar = 500 μm. f Representative heatmap image of social discrimination test. g Social discrimination test. (Control AAV, n = 10 mice; vCA1- Shank3-cKO, n = 10 mice; vmPFC-Shank3-cKO, n = 11 mice; NAcS-Shank3-cKO, n = 17 mice; LH-Shank3-cKO, n = 13 mice. Two-way mixed model ANOVA). h Social discrimination score. (Control AAV, n = 10 mice; vCA1- Shank3-cKO, n = 10 mice; vmPFC-Shank3-cKO, n = 11 mice; NAcS-Shank3-cKO, n = 17 mice; LH-Shank3-cKO, n = 13 mice. One-way ANOVA followed by Scheffe’s multiple comparisons test). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s. not significant. Data presented as mean ± SEM. See Supplementary Table 1 for exact p values. Source data are provided as a Source Data file.

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