Fig. 2: EV delivering Cas9/sgRNA RNPs edited EGFP gene of HEK293-EGFP in vitro. | Nature Communications

Fig. 2: EV delivering Cas9/sgRNA RNPs edited EGFP gene of HEK293-EGFP in vitro.

From: Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory in mice

Fig. 2

a Schematic illustration of the EGFP gene targeting Cas9/sgRNA RNPs containing EV production and transduction. b. Schematic illustration of the position of the EGFP gene targeting sgRNA on the EGFP gene. c Representative confocal microscopy images of HEK293-EGFP (upper panel) and EGFP-targeting EV (EV-#3) transduced HEK293-EGFP cells. GFP (green) expressed in the HEK293-EGFP cell and Cas9-fused CherryPicker (Magenta) signal were derived from EV. Scale bar = 50 μm. d Representative raw flow cytometry data of untreated HEK293-EGFP cells (left panel) and EGFP-targeting EV transduced HEK293-EGFP cells (right panel). e Quantification of EGFP negative cell ratio of the EGFP-targeting EV transduced HEK293-EGFP cells with varying concentrations (Control, 1/1–1/16: n = 6 samples. One way ANOVA followed by Dunnett’s multiple comparisons test). f Representative raw flow cytometry data of untreated HEK293-EGFP cells (left panel) and EGFP-targeting AAV transfected HEK293-EGFP cells (right panel). g Quantification of EGFP negative cell ratio of the AAV transfected HEK293-EGFP cells with varying concentrations (Control: n = 4 samples, 1/1: n = 6 samples, 1/2: n = 5 samples, 1/4: n = 6 samples, 1/8: n = 7 samples, 1/16: n = 6 samples. One way ANOVA followed by Dunnett’s multiple comparison test). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant. Data presented as mean ± SEM. Source data are provided as a Source Data file.

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