Fig. 3: EGFP-targeting EVs edit EGFP gene in NAcS of Drd1-EGFP mice in vivo. | Nature Communications

Fig. 3: EGFP-targeting EVs edit EGFP gene in NAcS of Drd1-EGFP mice in vivo.

From: Conditional knockout of Shank3 in the ventral CA1 by quantitative in vivo genome-editing impairs social memory in mice

Fig. 3

a Schematic illustration of the EGFP gene targeting EV injection in NAcS of Drd1-EGFP mice. b Representative confocal microscopy image of EV injected NAcS 24 hours after injection stained with anti-RFP (red, for Cas9 fused CherryPicker expression) and DAPI (blue). Scale bar = 1 mm. c. Representative confocal microscopy image of EV injected NAcS of a Drd1-EGFP mouse 5 weeks after injection, stained with anti-GFP (green, for EGFP) and DAPI. Macro view of EV-injected NAcS (upper panels), the EV-injected side (lower right panel), and the control injected side (lower left panel). Scale bar = 1 mm (upper panels); 200 μm (lower panels). d. Quantification of mean fluorescent intensity of control injected side and EV injected side (n = 4 samples. Paired t-test, two-tailed). e Quantification of relative ratio of mean intensity (EV injected site / Control site) in varied EV concentration (1/1–2/5: n = 4 samples. One way ANOVA followed by Tukey’s multiple comparison). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s. not significant. Data presented as mean ± SEM. Source data are provided as a Source Data file.

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