Fig. 3: Impact of bsaP on growth of bioB mutants and BioB expression.
From: Mycobacterial biotin synthases require an auxiliary protein to convert dethiobiotin into biotin
a Immunoblots for expression of BioB and PrcB (loading control) in M. tuberculosis and M. smegmatis. The entire blot is shown in Supplementary Fig. 11b. b, c Growth of M. smegmatis ΔbioBop (b) and E. coli ΔbioB (c) with and without extrabacterial biotin. d Immunoblots for expression of BioB and GAPDH (loading control) in E. coli. The entire blot is shown in Supplementary Fig. 11c. a, d Results represent at least three independent experiments. b, c Source data are provided as a Source data file.
