Fig. 4: Biochemical characterization.
From: Mycobacterial biotin synthases require an auxiliary protein to convert dethiobiotin into biotin
a BsaP is essential for the biotin synthesis catalyzed by BioB.tb, but not BioB.ec. b Progress curve of biotin synthesis catalyzed by BioB.tb (5 µM) with and without Bsap (5 µM). c Saturation curve of v0 vs [DTB]. The assay was performed under initial velocity conditions with a fixed saturating concentration of SAM (100 µM) while varying [DTB]. The KM and kcat values were measured by fitting the v0 vs [DTB] to Eq. 1. d Saturation curve of BioB.tb + BsaP showing the effect of increasing BsaP concentration on the overall rate of turnover. The KD of BioB.tb and BsaP interaction was measured by fitting the v0 vs [BsaP] to Eq. 2. a–d n = 3. Two-sided unpaired t test; ****p = 1.3537E-07, p = 0.2520 (ns); ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001 (a). Error bars, mean ± SD. Source data are provided as a Source data file.
