Fig. 1: Overall structure of the TMR-DN-bound RhoBAST aptamer.
From: Structural mechanisms for binding and activation of a contact-quenched fluorophore by RhoBAST
a Chemical structures of the conditional fluorophores of TMR-DN and SpyRho. b The previously predicted secondary structure of RhoBAST WT. c An updated secondary structure of RhoBAST (L2-U1 construct) from the crystal structure in this work. d A schematic representation of the secondary structure of RhoBAST highlighting long-range interactions and non-canonical base pairs. Thin lines with arrowheads and Leontis–Westhof symbols denote connectivity and base pairs, respectively. e Cartoon representation of the three-dimensional structure of the RhoBAST aptamer in complex with TMR-DN (red stick representation) and U1A protein (gray surface representation) in three orthogonal views. Two Mg2+ ions are shown as magenta.
