Fig. 3: Interactions between TssA proteins and their contractile sheath components.

Protein-protein interactions were assessed by bacterial two-hybrid (BTH), where bacteria appear blue upon interaction of fusion proteins, and white in the absence of interaction. a BTH interactions between P. aeruginosa TssA proteins and their respective sheath components, with TssB and TssC components produced alone and in combination. b BTH interactions between each TssA Nt1 domain with each sheath complex.