Fig. 4: Structural analysis of TssAS C-terminal domains (CTDs).
a SDS-PAGE of the purified TssA1 CTD, sample taken from the middle of the elution peak (Supplementary Fig. 4a). b SDS-PAGE of the purified TssA3 CTD, sample taken from the middle of the elution peak (Supplementary Fig. 4b). c Representative negative stain EM images of TssA1 CTD single particles. Scale bar represents 200 nm. d Representative negative stain EM images of TssA3 CTD single particles. e Representative 2D class averages of TssA1 CTD particles picked from cryo-EM images. Class average 1 is enlarged (below) with ring dimensions shown. f Representative 2D class averages of TssA3 CTD particles picked from negative stain EM images. Class average 1 is enlarged (below). Scale is not comparable between e and f. Source data are provided as a source data file.
