Fig. 2: Blood cytokine-receptor signaling and downstream effects. | Nature Communications

Fig. 2: Blood cytokine-receptor signaling and downstream effects.

From: Pulmonary maternal immune activation does not cross the placenta but leads to fetal metabolic adaptation

Fig. 2

A Maternal lung cytokine mRNA expression vs. maternal plasma protein levels. Y-axis shows maternal lung mRNA lipopolysaccharide (LPS) vs. Ctrl log2 fold change (log2FC). X-axis shows maternal plasma LPS vs. Ctrl log2FC of corresponding proteins. Cytokines are indicated by color: numbers indicate hours after instillation. Dotted lines show X = Y. B Maternal liver cytokine mRNA expression vs. maternal plasma protein levels. As in (A), but Y-axis shows maternal liver mRNA expression change. C Maternal plasma CCL20 abundance change vs. mRNA expression of Ccr6 in decidua and placenta. Y-axis shows maternal plasma CCL20 LPS vs. Ctrl log2FC. X-axis shows the average Ccr6 mRNA expression level in Ctrl decidua (dotted lines) or placenta (solid lines). Line colors and numbers as in (A). Subpanels show Ctrl and LPS treatment. D Maternal plasma IL-6 abundance change vs. decidua and placenta mRNA expression of Il6ra and Il6st. As in (C), but shows IL-6::Il6ra and IL-6::Il6st pairs. E mRNA expression of Il6ra and Il6st in decidua and placenta. Y-axis shows mRNA expression in decidua (gray) and placenta (white). Dots show samples and X-axis hours after instillation. Two-sided Mann–Whitney test P-values between distributions are shown, bold when P < 0.05. Subplots show Ctrl and LPS-treated mice. Receptor name on top. Boxplot center = median, box = interquartile range, whiskers = max 1.5*interquartile range from hinge; all data points shown. Sample sizes for each group/timepoint are shown in Il6ra subplots (same for Il6st). F Heatmap of the most LPS instillation-responding genes in the IL-6 pathway. Rows show genes. Columns show hours after instillation. Tissue on top. Cell color indicates LPS vs. Ctrl RNA-seq log2FC. G Socs3 gene expression change in paired decidua and placenta samples. Y and X-axes show Socs3 gene expression in the placenta and decidua. Dots show dam-fetus pairs. Dot shape indicates treatment. Color shows maternal plasma IL-6 concentration. Dotted lines indicate X = Y. Highlights show Socs3 upregulation in LPS-treated placenta. Source data in fig2_cytokines_conc.csv, fig1_limma_results_no_maternal_contrasts.csv and fig2_tpm_tibble.rds.

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