Fig. 6: Phosphoproteomics analysis of early placental response.

A Gene ontology (GO) analysis based on genes with changing phosphosites in placenta. Rows show GO terms, organized in functional themes as indicated on top of boxes. Columns indicate time after instillation (h). Dot opacity indicates significance (red color indicates FDR < 0.05), dot size indicates effect size. The analysis is based on proteins having one or more significant phosphosite changes, regardless of the direction of change. B Proteins and phosphosites with lipopolysaccharide (LPS)-induced phosphorylation increase at 2 h and decrease at 5 h. The left two heatmap columns show phosphosites for a given protein at 2 and 5 h, as indicated by the two first columns. Colors show average LPS vs. Ctrl log₂FC, where positive values represent a gain of phosphorylation in LPS vs. Ctrl. The two heatmap columns to the right show a change in expression level LPS vs. Ctrl log₂FC for the corresponding gene at 2 and 5 h. The last column shows the gene function category, based on gene ontology analysis. Displayed phosphosites have a significant (FDR < 0.05, see “Methods” section) change in phosphorylation state at 2 or 5 h, an LPS vs. Ctrl log₂FC > 0 at 2 h, and higher LPS vs. Ctrl log₂FC at 2 than at 5 h. Source data in folder fig6_phospho_placenta and fig1_limma_results_no_maternal_contrasts.csv.