Fig. 8: Analysis of fetal-liver lipid metabolism.

A LPS vs. Ctrl lipid abundance change at each timepoint. Heatmap is based on the top 50 most significantly lipopolysaccharide (LPS) vs. Ctrl changing lipid species (sorted by lowest FDR at any timepoint) clustered by Euclidean distance. Lipids are annotated by class (right). Bold typeface indicates 22:6 side chains. Heatmap cell colors indicate LPS vs. Ctrl log₂ fold change (log₂FC). Columns right of the heatmap show lipid species class as colored rectangles and name. For class color schema and abbreviations, see the legend to the right. B Abundance of 22:6 lipid species in fetal liver. The Y-axis shows the average peak intensity. X-axis indicates time after instillation (h). Numbers on top are P-values from Mann-Whitney two-sided tests, comparing LPS vs. Ctrl. Dots within density plots indicate distribution means. Color indicates treatment. C Network and abundance changes of selected lipids and lipid-processing enzymes. Arrows show conversions of lipids or lipid classes (rectangular boxes: bold typeface indicate 22:6-containing species). Selected enzymes for conversion are also shown (rounded boxes). Boxes are colored by whether the lipid or enzyme is up, down, or unchanged over time following LPS instillation. Examples of temporal regulation on lipid species levels are shown as callouts: in these, Y-axis shows average abundance (peak intensity), X-axis shows time after instillation. Error bars show the standard error of the mean. Arrows ending outside the cell boundary denote putative export of lipids into the bloodstream by lipoproteins. Sample size for each group/timepoint shown on top left plot (same in all plots, colored by treatment type as above). Source data in folder fig8_9_lipidomics and fig1_limma_results_no_maternal_contrasts.csv.