Fig. 5: GalNAc-T3 and T7 inhibit replication of the omicron variant of SARS-CoV-2 in Calu-3 cells. | Nature Communications

Fig. 5: GalNAc-T3 and T7 inhibit replication of the omicron variant of SARS-CoV-2 in Calu-3 cells.

From: Sequential glycosylations at the multibasic cleavage site of SARS-CoV-2 spike protein regulate viral activity

Fig. 5

a Western blot analysis of the assembly of SARS-CoV-2 VLPs with alpha or omicron spike protein in HEK293T WT and GALNT3/T7 KI cells. VLPs were assembled and detected as in Fig. 3. VLPs with Wuhan spike protein were produced in parallel as a control. b Viral titers from Calu-3 cells infected with SARS-CoV-2 omicron variant (BA.1) at an MOI of 0.1, with or without co-expression of GalNAc-T3 and T7. c Viral titers from Calu-3 cells infected with SARS-CoV-2 omicron variant (BA.1) at an MOI of 0.1 with or without overexpression of GalNAc-T7. For (b, c) data are presented as mean values ± SEM (n = 3 biologically independent experiments), and two-tailed P values are calculated by unpaired Student’s t test and displayed above the compared means. d Western blot analysis of S protein and N protein of the omicron virions from Calu-3 cells 48 hours post infection (HPI), with or without co-expression of GalNAc-T3 and T7. e Western blot analysis of S protein and N protein of the omicron virions from Calu-3 cells 48 h post infection (HPI), with or without overexpression of GalNAc-T7. For data (a, d, e), the results here are representative blots from three independent experiments. Source data are provided as a Source Data file.

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