Fig. 2: Tet2 is required for correct myeloid cell priming.

a Plot showing the fold change in the accessibility of chromatin peaks comparing WT and Tet2 KO GMP cells. Cells belonging to clusters 1,4,7,10,13 for GMP samples were included. Significantly upregulated peaks (FDR < 0.01) are colored in red while significantly downregulated peaks are colored in blue. b Z-score accessibility of differential peaks in GMPs from Fig. 2a measured per-cell relative to the entire dataset. Genotype is indicated. c TF motif accessibility comparing WT and Tet2 KO GMP cells measured in differential peaks regions (Left: upregulated, Right: downregulated). d Cumulative accessibility of differential upregulated (left) and downregulated (right) GMP peaks from Fig. 2a plotted on UMAP coordinates. e Plot showing the mean difference in GMP chromatin signatures from Fig. 1 comparing WT and Tet2 KO cells. Level of significance is color coded. f Pathway enrichment analysis (performed using Metascape¹¹⁵) of upregulated (left) and downregulated (right) transcripts in the indicated cell populations. Significance level is color coded. p-values are calculated based on the two-sided cumulative hypergeometric distribution, no adjustment for multiple comparisons is reported. g Functional assessment of WT and Tet2 KO primary monocytes and neutrophils, showing % phagocytosis with LPS stimulation (left), production of ROS with PMA stimulation (middle), production of ROS without PMA stimulation (right) n = 4 independent mice, two-tailed paired t-test; exact p-values are indicated on the figure. Data are presented as mean values +/- SEM. Genotype is color coded. Source data are provided as a Source Data file.