Fig. 5: The deficiency in HOPS complex leads to reduced mechanical penetration of hyphae.
a Vacuoles in hyphae of C. albicans SC5314 cultured in liquid RPMI1640 + 10% FBS medium for 90 min and solid Spider media for 48 h. Scale bars = 5 μm. b Imaging of Yvc1-GFP strains in hyphae sliced from Spider agar. Scale bars = 10 μm. c Vacuoles stained by VacuRed in hyphae scraped from Spider agar. Scale bars = 5 μm. d The fusion process of vacuoles during hyphal growth in C. albicans. SC5314 were cultured in liquid RPMI1640 + 10% FBS medium at 37 °C for 1 h, and stained with VacuRed. The vacuoles in hyphae were observed every 30 s. A 90 s time period is shown. Scale bars = 5 μm. The representative images shown are from 3 biological repeats (a–d). e, f Vertical sections of hyphal colonies on solid agar. Width (W) and depth (D) (mm) were quantified by Image J. n = 4 colonies. Scale bars = 1 mm. g Top view of hyphal colony on Spider + 10 mM CaCl2 solid plates containing different concentrations of agar. h The hyphae invasion ratio calculated by PHOPS-complex-deficient mutants/PSC5314 in the same concentrations of agar. Parameters C and P of hyphal colonies in (g) were measured by Image J. n = 13 colonies. i Top view of hyphal colony on RPMI1640 + 10% FBS solid plates containing different concentrations of agar. Three biological replicates were carried out. One of the representative images are shown (e, g, i). j The hyphae invasion ratio calculated by PHOPS-complex-deficient mutants/PSC5314 in the same concentrations of agar. Parameters C and P of hyphal colonies in i were measured by Image J. n = 7 colonies. Data were presented as mean ± SD (h, j). Two-tailed unpaired t-test (f, h, j). Source data are provided as a Source Data file.
