Fig. 7: Characterization of primary human hepatocyte organoid cultures.

a Representative low- and high-magnification brightfield images of expanding PHH organoids (passage 4) in the IL6+FXRa condition. Scale bar = 400 μm (low mag) and 100 μm (high mag). b Representative image of H&E staining of PHH organoids. Scale bar = 75 μm. c Representative immunofluorescence staining for Ki-67 of PHH organoids. Scale bar = 100 μm. d, e Representative immunofluorescence staining of PHH organoids for (d) ALB, AFP, CK19, and CK7 and (e) A1AT and HNF4A, CYP3A4, and ZO1. Scale bar = 100 μm (d, e). f Single-cell profiling of PHH organoids. g UMAP plots of the indicated markers. h Single-cell profiles of the FH and PHH organoids (originating from a single library, with the two cultures distinguished based on genotype demultiplexing). i UMAP plots of the indicated fetal and adult hepatocyte markers in the combined single cell datasets. j Schematic illustrating the dynamics underlying human hepatocyte growth and differences between fetal and adult. a–e Representative of characterization of n = 2 expanding PHH organoid cultures.