Fig. 5: Elevated IFN signaling is negatively associated with Spike-specific CD4+ T cell responses.
From: Sustained IFN signaling is associated with delayed development of SARS-CoV-2-specific immunity
A Representative flow cytometry gates used to detect Spike-specific CD4+ T cells following 15 h peptide stimulation. Boolean OR gating strategy used. B–E Correlations between absolute counts of Spike-specific CD4+ T cells with (B) ssGSEA IFN score; (C) ssGSEA COVID-19 severity score; (D) absolute counts of RBD-specific B cells or (E) absolute counts of RBD-specific plasma cells. F Comparison of absolute counts of Spike-specific CD4+ T cells per patient cluster. G Representative flow cytometry gate used to detect Spike-specific CD8+ T cells following 15 h peptide stimulation. H, I Correlations between absolute counts of Spike-specific CD8+ T cells with (H) ssGSEA IFN score and (I) ssGSEA COVID-19 severity score. J Comparison of absolute counts of Spike-specific CD8+ T cells per patient cluster. K Correlation between the absolute counts of both Spike-specific T cell populations. n for cluster 1 = 11; 2 = 11; 3 = 9; 4 = 10. B–E, H, I, K Two-tailed Spearman correlation. F, J Kruskal-Wallis with Dunn’s multiple comparison tests. Adjusted p-values are shown. Medians are shown in bar charts. Source data are provided as Source_Data_File.xlsx.
