Fig. 4: Differences in nucleosome dynamics and organization at nuclear periphery and nuclear interior.

A Example distance to nuclear edge image. Scale bar = 1000 nm (B) Box plot of the diffusion coefficient as function of distance from nuclear edge. The plot follows the same convention as Fig. 2B. C Box plot of the anomalous alpha exponent as a function of distance from nuclear edge. The plot follows the same convention as (B). D Violin plot of voxel distance from the nuclear edge within each chromatin density class. Boxes indicates the inter-quartile range. White dot indicates median, and bars indicate 1.5 x the limits of inter-quartile range. E (Left) Example deconvolved image. Dashed line indicates 500 nm from the nuclear edge. (Middle, Right) Example CDC images at the nuclear interior and periphery. Scale bar = 500 nm (F) Box plot of the diffusion coefficient across different chromatin classes at the nuclear interior (Gray) and nuclear periphery (White). The plot follows the same convention as (B). G Box plot of the anomalous alpha exponent, the plot follows the same color assignments and convention as (F). H Box plot of the diffusion coefficient for HaloTag-NLS across different chromatin density classes at the nuclear interior and nuclear periphery. The plot follows the same color assignments and convention as (F). I Box plot of the anomalous alpha exponent for HaloTag-NLS across different chromatin density classes at the nuclear interior and nuclear periphery. The plot follows the same color assignments and convention as (F). J Box plot of the estimated fractal dimension across different chromatin density classes at the nuclear interior and nuclear periphery. The plot follows the same color assignments and convention as (F). Data from (B–D), (F), and (G) are from n = 88 cells across 8 independent biological replicates. Data from (H) and (I) are from n = 37 cells across 4 independent biological replicates. Data from (J) are from n = 54 cells across 3 independent biological replicates. Box plots in (F–I) include only points from line fits to data that include at least 250 trajectories, and “NSD” indicates not sufficient data. Box plot in (J) includes only points from line fits with r² > 0.95.