Fig. 6: Measurements of the relationship between nuclear functions and nucleosome properties.

A Schematic of different perturbations targeting gene transcription. B Box plot of diffusion coefficient in different chromatin density classes under control (blue), α-amanitin (orange), DRB (green) and PladB (purple). WC stands for whole cell. C, D Box plot of diffusion coefficient and anomalous alpha exponent in different chromatin density classes under control (blue), actinomycin D (red). E Schematic of Trichostatin A (TSA) perturbation on histone deacetylase activity. F Box plot of diffusion coefficient in different chromatin density classes under control (blue) and TSA (pink). G Point plot displaying the distribution of nuclear voxels in different chromatin classes under control (blue), ActD (red) and TSA (pink) conditions. The central dot indicates the mean proportion of voxels in given class for all cells. The vertical line indicates the standard deviation. H Box plot of estimated fractal dimension from super-resolution images of different chromatin classes under control (blue), ActD (red) and TSA (pink) conditions. Data from (B), (C), (D), (F) and (G) are from n = 88 cells across 8 replicates (control), n = 52 cells across 3 replicates (α-amanitin), n = 46 cells across 3 replicates (DRB), n = 49 cells across 3 replicates (PladB), n = 41 cells across 3 replicates (ActD) and n = 60 cells across 3 independent replicates (TSA). Data from (H) are from n = 54 cells (control), n = 54 cells (ActD), and n = 60 cells across 3 replicates (TSA). All replicates above were independent biological replicates. Box plots in (B–D), (F) and (H) follow same conventions as box plot in Fig. 2B.