Fig. 2: Reproducibility of replicate AEMS peptide ejections over 5.25 h.
From: Acoustic ejection mass spectrometry empowers ultra-fast protein biomarker quantification
A The measurement of a peptide across 384 sample wells at the acquisition rate of 1.5 secs per well requires 10.5 min to acquire. B This measurement of the complete plate was repeated 30 times (acquiring 11490 sample ejections in total) and the peak area and peak area ratio percent coefficient of variation (%CV) were determined for each plate run. The %CV for the peak areas for the light and heavy peptides (Light AYNVTQAFGR (blue), heavy AYNVTQAFGR (orange)) were between 4.6 and 6.4% and the peak area ratios (summed L/H peak area ratio (green)) were between 6.2 and 7.2%.
