Fig. 2: Long-term SR imaging of rapid and photo-sensitive bioprocesses via ZS-DeconvNet.
From: Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopy
a Representative SR images reconstructed by ZS-DeconvNet of F-actin cytoskeleton and myosin-II in a COS-7 cell co-expressing mEmerald-lifeact and mCherry-myosin-IIA. Comparisons of raw noisy TIRF image and images processed by RL deconvolution, DeepCAD-based deconvolution and ZS-DeconvNet are displayed. b Two-color time-lapse SR images enhanced via ZS-DeconvNet showing the coordinated dynamics of F-actin (cyan) and myosin-II (yellow) over the whole spreading process after placing a COS-7 cell onto a coverslip (Supplementary Video 2). c, d Two-color time-lapse SR images enhanced via ZS-DeconvNet of F-actin and myosin-II in a crawling COS-7 cell showing that myosin-II preferentially concentrates to the rear of the cell (outlined by yellow dashed lines in d), opposite to crawling direction (indicated by the white arrows in d) (Supplementary Video 3). e Representative SR image generated via ZS-DeconvNet of recycling endosomes (REs, green) and late endosomes (LEs, magenta) in a gene-edited SUM-159 cell endogenously expressing EGFP-Rab11 and mCherry-Lamp1 (Supplementary Video 4). f Typical trajectories of RE (top) and LE (bottom) movements showing the rapid directional motility of RE, and the bidirectional nature of LE. g Comparisons of the speed, displacement, and traveling time between Lyso/LEs and REs, and quantification of the residence time of REs near their exocytosis sites before fusing with plasma membrane (n = 505 tracks for REs and n = 230 tracks for LEs). A small number of data points exceeding transportation time of 150 s or displacement of 60 μm were not displayed for better presentation of the distributions. Center line, medians; limits, 75% and 25%. Statistical significance was determined using unpaired Mann-Whitney test (p = \(1.38\times {10}^{-7}\), \(5.65\times {10}^{-35}\), and \(6.26\times {10}^{-40}\) for tests of the moving speed, transportation time, and displacement, respectively). ****p < 0.0001. Source data are provided as a Source Data file. h Time-lapse images illustrate the directional movement of a RE in rod-like shape, and the subsequent fusion with plasma membrane. i Time-lapse images illustrate three LEs tether each other and co-migrate for certain distance before splitting into individual LEs. Scale bar, 5 μm (a, c, d), 2 μm (zoom-in regions in a), 8 μm (b), 3 μm (e), 0.5 μm (zoom-in region in e), 1 μm (g, f, i).
