Fig. 2: Bar-seq of A. fumigatus identifies YakA as a potential antifungal target.

A Heatmap of fitness for each protein kinase null mutant relative to control conditions (n = 5). Relative fitness values was calculated by DESeq2, comparing barcode counts obtained from 20 h fRPMI1640 (pH 7, 37 °C, shaking 130 rpm) cultures with those supplemented with 1.5 mM H₂O₂ (to induce oxidative stress), voriconazole 0.15 mg/l, olorofim 0.002 mg/l, pH adjusted to pH4 or pH8 or with modified culture temperature (48 and 30 °C). and different iron concentrations (Fe−, BPS, 30 μM, 300 μM, 5 mM). The mouse data fitness values was calculated by DESeq2, comparing barcode counts obtained from the input pool with the infected samples. The heatmap was generated with Pheatmap, full linkage clustering on rows and columns (cutree_cols = 2). Source data are provided as a Source Data file. B Dry weight was measured after 16 h culture in HMM, with and without 0.5 mg/l itraconazole, at 37 °C shaking 130 rpm. Data are presented as mean values with SEM. Statistical difference was assessed by using one-way ANOVA (n = 2 or 3 biological replicates). Source data are provided as a Source Data file. C 1000 spores of MFIG001, ∆yakA and the reconstituted isolate (yakA+) were spot inoculated on HMM agar without iron and with 1 µM iron added and incubated for 72 h at 37 °C. Representative pictures are shown. D Survival curves in a Galleria mellonella model of aspergillosis. Statistical difference was assessed by Kaplan–Meier survival analysis and p values between groups are shown on the graph. E Survival in a leukopenic mouse model of aspergillosis (n = 11). Statistical difference was assessed by Kaplan–Meier survival analysis p values between groups are shown on the graph.