Fig. 3: Detailed phenotypic analysis of the ∆yakA isolate.

A 10³ spores of the isotype control MFIG001, ∆yakA, yakA+ were spotted on HMM + 30 µM Fe with increasing concentration of agarose (0.5–6%) and imaged after 72 h incubation at 37 °C. Representative images are shown. B Representative histological sections of lesions found in mouse lungs infected for 3 days with MFIG001, ∆yakA and yakA+. H&E and GMS stains were performed on neighbouring sections. C The hyphal length within histological sections (n = 15 biological replicates) was measured. Data are presented as mean values with SEM. Statistical differences were assessed by one-way ANOVA. Source data are provided as a Source Data file. D Recovery from cytorrhysis after addition of glycerol to liquid cultures. Liquid cultures were grown for 16 h at 37 °C in an Ibidi imaging chamber. 1.8 M glycerol was added and images were taken every 150 s. The % cytoplasm compared to the total cell size was measured for individual septal compartments (n = 10 biological replicates) using Fiji. Data are presented as mean values with SEM. Source data are provided as a Source Data file.