Fig. 1: The nanoinjection platform.
A SICM integration into the platform. The position of the nanopipette is controlled by the SICM through a piezoelectric actuator. B The quantitative nanoinjection procedure. The nanopipette approaches the surface of the cell membrane through the spatial control of the SICM, then the nanopipette is moved downward by a predefined distance to penetrate the cell, finally, the delivery of materials will be triggered by electrophoretic forces via the application of a suitable voltage. During delivery, the current is monitored in real-time, and the translocation of a single analyte disrupts the current baseline and appears as a peak, quantifying the number of peaks and thus revealing the number of molecules delivered to the cell.
