Fig. 4: The quantitative nanoinjection of α-synuclein fibrils into rat primary cortical neurons.

A A representative image of the α-synuclein fibrils and B their associated length distribution of 69 ± 2 nm (standard error of the mean, 628 fibrils traced). C The primary neuron before and after the nanoinjection of the α-synuclein fibrils. D A snapshot of the current trace (100 seconds) during the nanoinjection. Based on peak counting, a total of 153 α-synuclein fibrils were nanoinjected into the cell. The experiments were repeated three times, and the replicates can be found in the Supplementary Information. Source data are provided as a Source Data file.