Fig. 4: Cell-type-specific isoforms and their characteristics.
From: Long-read sequencing for 29 immune cell subsets reveals disease-linked isoforms
A Unsupervised hierarchical clustering based on isoform ratios that were the top 5000 large expression variances among cell types. The colored bars indicate the cell lineages. B Cell-type-specific expressed isoforms. Column-wise Z scores of normalized counts are plotted. Representative gene symbols related to specific isoforms are annotated at the top. Full lists of cell-type-specific isoforms are provided in Supplementary Data 5. C The length of the 5′-UTR, ORF, and 3′-UTR (top) and the proportion of unique TSS, last exon, insertion of repetitive elements in splicing junctions, and TTS (bottom) compared between cell-type-specific isoforms (n = 2575) and others (n = 156,794). The significance of comparison is as follows: ****, nominal p < 0.0001; ***, nominal p < 0.001; **, nominal p < 0.01; *, nominal p < 0.05 by two-sided Wilcoxon test. The center of each box plot is the median value, the edges of the box represent the first (Q1) and third (Q3) quartiles, and the minimum/maximum whisker values are calculated as Q1/Q3 -/ + 1.5 × the interquartile range (IQR). D Enrichment of each RBP motif according to gene regions compared between cell-type-specific isoforms and others. Representative significant RBPs (FDR < 0.01) are annotated at the top. E Example of a specifically expressed isoform transcribed from the NLRP1 locus. The collapsed gene structure registered in GENCODE is shown at the bottom. Arrows indicate the direction of transcription in the genome. F Isoform ratio in NLRP1.
