Fig. 1: Experimental design and overall pre- and post-timepoint comparisons.
From: Spatial multi-omics of human skin reveals KRAS and inflammatory responses to spaceflight
a Experimental design and workflow with representative tissue staining images (created with BioRender.com), b Uniform Manifold Approximation and Projection (UMAP) of all ROIs collected, c Volcano plot of overall post- vs. pre-spaceflight DEGs (using DESeq2 method), d Pathway enrichment analysis comparing DEGs from pre- and post-spaceflight skin tissues, visualizing normalized enrichment scores of MSigDB Hallmark pathways, and e Cell proportion comparisons between pre- and post-spaceflight samples (ns non-significant, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 by Wilcoxon test, two-sided; boxplot shows median/horizontal line inside the box, the interquartile range/box boundaries, whiskers extending to 1.5 times the interquartile range, and outliers as individual points outside the whiskers; exact p values are included in the Source Data). Source data are provided as a Source Data file.
