Fig. 5: Identification of HNF1A-bound targets in pancreatic endocrine cells highlight several target genes regulated by HNF1A.

a Topmost gene ontology (GO) biological processes (BP) found in HNF1A ChIP-Seq targets in human islets. Analysis and visualization of GO data is based on the ChIPseeker R package (see Methods). b Venn diagram showing overlaps in HNF1A-bound target genes in D20 EPs and human islets based on ChIP-Seq peaks within 10 kb of the transcription start site (TSS) (number of total peaks with no filtering shown in brackets). Table provides a consensus list of the beta cell target genes identified. c IGV tracks showing HNF1A ChIP-Seq peaks that map to the nearest genes in selected loci in D20 EPs and human islets. The scale used to visualize peaks in IGV is indicated on the right side of each track. The chromosomal location near the peak region is indicated. d Gene expression analysis of selected target genes in EndoC-βH1 cells with HNF1A siRNA-mediated knockdown (n = 3). * indicates p < 0.05, ** indicates p < 0.01 relative to si-NT (non-targeting control) using paired two-tailed Students’ t test. e Transactivation activity of GPR39 promoter in EndoC-βH1 cells (n = 3). * indicates p < 0.05 relative to Empty si-HNF1A control in the presence of the GPR39 promoter; $ indicates p < 0.01 relative to WT, based on one-way ANOVA with Tukey’s post-hoc test. Data are presented as mean ± SD. Each data point represents one independent experiment. Source data and exact P values are provided in the Source Data file.