Fig. 7: Investigation of the effects of HNF4A T2D risk variant rs1800961 on gene regulation using FLAG ChIP-Seq. | Nature Communications

Fig. 7: Investigation of the effects of HNF4A T2D risk variant rs1800961 on gene regulation using FLAG ChIP-Seq.

From: HNF4A and HNF1A exhibit tissue specific target gene regulation in pancreatic beta cells and hepatocytes

Fig. 7

a Western blot analysis of cell lysates from EndoC-βH1 cells stably overexpressing FLAG-tagged HNF4A WT and T2D variant constructs. b Immunofluorescence microscopy images showing nuclear localization of FLAG-tagged HNF4A WT and T2D variants overexpressed in EndoC-βH1 cells, with constitutive GFP signal from the vector in green, FLAG-tagged protein in red and DAPI in blue. Enlarged images from the insets are shown in the last panel. Scale bar indicates 100 µm. c HNF4A FLAG ChIP-qPCR fold enrichment at the HNF1A promoter in the stable EndoC-βH1 cell lines (n = 3). * indicates p < 0.05 relative to GFP control based on one-way ANOVA with Dunnett’s post-hoc test. d Representative HNF4A motif identified in the ChIP-Seq samples and the total number of ChIP-Seq peaks identified in each sample based on q value cut-off of 0.1. e Peak count frequency profile of HNF4A FLAG ChIP-Seq peaks that map within 1 kb of the transcription start site (TSS) across the EndoC-βH1 stable lines and the corresponding genomic feature distributions. Data are presented as mean ± SD. Each data point represents one independent experiment. Source data and exact P values are provided in the Source Data file.

Back to article page