Fig. 6: Positive cooperatively sequential binding mechanism.
From: Heliorhodopsin-mediated light-modulation of ABC transporter
a Thermodynamic parameters of OcHeR for OmrDE from ITC analysis in Table 2 and Supplementary Figs. 12 and 13. ITC analysis of OcHeR R233Q for OmrDE was performed using a sequential binding site model: R233Q-1, first binding; R233Q-2, second binding. Measurements were conducted in an independent experimental group (n = 2 to 5). data are presented as mean value ± SEM. b This mechanism is assumed by OcHeR with IFnarrow conformation of OmrDE in the absence of light, which is based on thermodynamic parameters in ITC analysis. Protein structures of OcHeR, OmrD, and OmrE are indicated in pink, cyan, and yellow, respectively. Attractants and repellents are indicated by red and blue arrows, respectively.
