Table 1 Steady-state enzyme kinetic parameters for NBDs in OmrDE with and without OcHeR
From: Heliorhodopsin-mediated light-modulation of ABC transporter
Omr | OcHeR | Light (532 nm) | Vmax (µM/min) | Km (mM, ATP) | kcat (s−1) | kcat/Km (M−1 S−1) |
|---|---|---|---|---|---|---|
DE | – | – | 17.6 ± 5 | 19.2 ± 12.3 | 0.293 ± 0.082 | 20.6 ± 9.3 |
DEm | – | – | 5.1 ± 0.7 | 27.6 ± 10.5 | 0.086 ± 0.011 | 3.7 ± 1.5 |
DE | WT | – | 18.3 ± 6.9 | 10.4 ± 7 | 0.304 ± 0.115 | 37.7 ± 13.7 |
DE | WT | + | 20.7 ± 2.2 | 6.3 ± 2 | 0.344 ± 0.037 | 58.4 ± 11.3 |
DE | R102Q | – | 18.2 ± 0.7 | 14.4 ± 1.8 | 0.303 ± 0.012 | 21.4 ± 2.5 |
DE | R102Q | + | 19.5 ± 1.7 | 18.1 ± 1.8 | 0.325 ± 0.016 | 18.1 ± 1.7 |
