Table 1 Kinetic parameters of enzymes used in [19F]FDT synthesis
From: Distributable, metabolic PET reporting of tuberculosis
Enzyme | Substrate | KM (mM) | kcat (s-1) | kcat/KM (mM-1s-1) |
|---|---|---|---|---|
OtsA a | UDP-Glc | 1.7 ± 0.3 | 34 ± 1 | 20 |
OtsA a | G6P | 7.3 ± 0.6 | 34 ± 1 | 4.7 |
TreT P. horikoshii b | UDP-Glc | 4.6 ± 0.8 | 25 ± 2 | 5.5 |
TreT P. horikoshii b | Glc | 8.1 ± 0.6 | 13.6 ± 0.4 | 1.7 |
TreT T. tenax c | UDP-Glc | 2.3 ± 0.7 | 13.5 ± 1.4 | 6.2 |
TreT T. tenax b | Glc | 5.0 ± 1.3 | 10.3 ± 1.3 | 2.1 |
OtsAB-fusion d | UDP-Glc | 7.2 ± 5.7 | - | - |
OtsB e | T6P | 3.6 ± 0.8 | 286 ± 34 | 79 |
OtsASf b | UDP-Glc | 1.4 ± 0.2 | 63.5 ± 2.5 | 45.3 |
OtsASf b | G6P | 4.9 ± 0.5 | 57.5 ± 2.3 | 11.9 |
OtsBSf e | T6P | 2.8 ± 0.7 | 740 ± 96 | 264 |