Fig. 6: scRNA-Seq reveals CD16- NK cells express high IL-18 transcripts in acute MIS-C patients. | Nature Communications

Fig. 6: scRNA-Seq reveals CD16- NK cells express high IL-18 transcripts in acute MIS-C patients.

From: Enhanced CD95 and interleukin 18 signalling accompany T cell receptor Vβ21.3+ activation in multi-inflammatory syndrome in children

Fig. 6

ScRNA-seq analysis of paired PBMC samples from five children with acute MIS-C and at follow-up (approximately one month later). A PBMC UMAP projection showing annotated cell populations B Differential cell abundance test result from miloR analysis, comparing acute and follow-up groups. Significantly changed clusters were colour indicated. C Distribution of TCR Vβ21.3+ and − T cells in different CD4 T subtypes. D selected M1, M2 marker expression in acute and follow-up monocyte populations (including the 4 different monocyte subtypes). E Expression of IL1B and IL-18 mRNA transcripts in the UMAP. F Violin plots of IL-18 mRNA levels in different PBMC populations from MIS-C patients at the acute or follow-up stage. There was a significant change in IL-18 mRNA levels for the CD16- NK population when comparing admission and follow-up samples by Wilcoxon’s tests (q value = 3.81E-06). G Intracellular staining of IL-18 in fixed blood samples. Left: staining in an infectious disease sample; right: example in an MIS-C (acute) sample. H Cell abundance comparing PBMC samples from healthy and MIS-C follow-up children. The healthy children PBMC data are from published dataset2. FC fold change, nc non-classical, cm central memory.

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