Fig. 1: Biosynthesis of RiPPs such as the class II lanthipeptides using the UniBioCat platform.
From: Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides
a Schematic diagram of the UniBioCat framework for cell-free biosynthesis of mature RiPPs from DNA as direct inputs. Created with BioRender.com. b Comparison of the RiPPs production timeline between UniBioCat and cell-based in vivo systems. c Overview of the UniBioCat workflow. By adding DNA templates of a RiPP biosynthetic pathway, UniBioCat enables in vitro transcription and translation to synthesize precursor peptide and modification enzyme(s). The enzyme(s) then modify the precursor peptide in situ for maturation, forming the final RiPP product. This integrative process can also be used to rapidly generate mutated RiPPs library. UniBioCat offers a fast and streamlined platform for RiPPs biosynthesis, engineering, and discovery. Created with BioRender.com.
