Fig. 4: UniBioCat enables discovery and biosynthesis of uncharacterized lanthipeptides.
From: Cell-free biosynthesis and engineering of ribosomally synthesized lanthipeptides
a Genome mining, cell-free biosynthesis, and MALDI-TOF-MS identification of uncharacterized lanthipeptides. Amino acid sequences of Lan 1 - Lan 12 are obtained from NCBI under the accession numbers shown in the brackets. The leader peptide of SboA is fused to the N-terminus of each selected core peptide, allowing the two biosynthetic enzymes (SboM and SboT) to produce mature lanthipeptides (e.g., Lan 1 - Lan 10). Note that in (a) all lanthipeptide sequences are presented with one additional amino acid alanine at the N-terminus of the final peptide, which is indicated with the [M + H]+ ions. b Antimicrobial activity assay showing ΔOD600 profiles of S. aureus RN4220 growth treated with cell-free synthesized products including three representative new lanthipeptides (i.e., Lan 5, Lan 7, and Lan 10; see Supplementary Fig. 16 for the activity assay of all tested lanthipeptides). Note that cell-free reaction mixture is used directly for the assay without purification of each lanthipeptide. NC, negative control without gene templates in the cell-free reaction; Kan, kanamycin (25 μg/mL) as a positive control. Data shown representative of three independent experiments (n = 3). In (b), data are presented as mean±s.d. of two independent experiments (n = 2). Source data are provided as a Source Data file.
