Fig. 4: Astrocytic ALKBH5 deletion increases m6A levels and alters mPFC epitranscriptome.
From: Astrocytic ALKBH5 in stress response contributes to depressive-like behaviors in mice
a Transcriptome-wide distribution of m6A peaks of control samples. b Number and percent of m6A peaks. c Consensus motif of m6A sites identified in m6A peaks of control samples. d Volcano diagram showing overlap of genes with significant changes in mRNA expression in the Astrocyte cKO mice and Ctrl. e Clustering and heatmaps showing mRNA levels in the mPFC Astrocyte cKO and Ctrl mice. Relative mRNA expression level is represented in color. Only m6A genes with enrichment fold >1 in controls and significant differences in mRNA expression between the experimental and control groups. f GO analysis of mRNAs with reads per kilobase of transcript, per million mapped reads (RPKM) ratio <1 and p < 0.05. g KEGG analysis with FC >1 and p < 0.05. h m6A modification on SLC1A2 gene. Two-tailed Fisher’s exact test and FDR corrected p < 0.05 were considered significant (d–g). Two-sided Fisher’s exact test with adjustments for multiple testing (c). See Supplementary Data 4 for statistical details.
