Fig. 8: SAMe produces antidepressant-like behaviors.
From: Astrocytic ALKBH5 in stress response contributes to depressive-like behaviors in mice
a Model of the SAMe function in m6A modification. b ELISA analysis of SAMe levels in the plasma of MDD patients and healthy individuals. (Ctrl, n = 21; MDD, n = 69 samples). c SAMe levels in the plasma of the mice with LPS-induced depression. (n = 6 mice per group). d, e SAMe levels in the mPFC of mice with LPS-induced depression (d n = 6 mice per group) and Sus, Res, and Ctrl mice after CSDS (e n = 5 mice per group). f m6A levels in the plasma of C57BL/6J mice treated with SAMe (300 μg mL−1) or vehicle. (n = 7 mice per group). g MeRIP-qPCR of GLT-1 m6A levels in the mPFC of C57BL/6J mice treated with SAMe (300 μg mL−1) or vehicle. (n = 3 mice per group). h–k Statistics analysis of C57BL/6J mice treated with SAMe or vehicle in FST (1 W) (h n = 10 mice per group), FST (3 W) (i n = 12 mice per group), SI test (j Ctrl, n = 7; SAMe, n = 8 mice), EPM (k n = 10 mice per group). l Schematic of the experimental paradigm. m, n Statistics analysis of OE-Alkbh5 and Ctrl mice treated with SAMe (300 μg mL−1) or vehicle in FST (m), SI test (n). (Ctrl, n = 8; OE, n = 7; OE-SAMe, n = 7 mice). All data were presented as the mean ± SEM. Two-sided unpaired t-test (b–d, f–i, k), one-way (e, m, n), two-way (j) ANOVA with Bonferroni’s multiple comparisons test. *p < 0.05; **p < 0.01; n.s. no significance. Source data are provided as a Source Data file. See Supplementary Data 4 for statistical details.
