Table 1 Target-specific nanobody families derived from immunized LamaMice
From: Generation of nanobodies from transgenic ‘LamaMice’ lacking an endogenous immunoglobulin repertoire
F# | clone | Strain | Target | Var | SHM | V | D | J | CDR3 | KD (nM) |
|---|---|---|---|---|---|---|---|---|---|---|
1 | 8-A112-1 | TE02 | AAV8 | 3 | 3–8 | 5 | 2 | 6 | 14 | nd |
2 | 8-A158-3 | TE02 | AAV8 | 5 | 5–8 | 5 | 6 | 4 | 13 | nd |
3 | 8-A25-2 | TE02 | AAV8 | 1 | 10 | 2 | 6 | 4 | 13 | nd |
4 | 8-A110-3 | TE02 | AAV8 | 1 | 6 | VH | 7 | 4 | 7 | nd |
5 | 8-A157-3 | TE02 | AAV8 | 3 | 3–4 | 5 | 6 | 4 | 14 | nd |
6 | E04 | TE03 | AAV8 | 5 | 4–11 | 5 | 6 | 4 | 14 | nd |
7 | B07 | TE03 | AAV8 | 5 | 5–9 | 5 | 6 | 4 | 14 | nd |
8 | E10 | TE03 | AAV8 | 1 | 8 | 5 | 2 | 6 | 14 | nd |
9 | D03 | TE03 | AAV8 | 1 | 15 | 5 | 1 | 4 | 13 | nd |
10 | D10 | TE03 | AAV8 | 3 | 0–3 | 5 | 2 | 6 | 13 | nd |
11 | F04 | TE03 | AAV8 | 5 | 6–11 | 5 | 6 | 4 | 13 | nd |
12 | E08 | TE03 | AAV8 | 1 | 11 | 6 | 6 | 4 | 12 | nd |
13 | C02 | TE03 | AAV8 | 4 | 8–11 | 5 | 6 | 4 | 10 | nd |
1 | 8SG-G1 | TE02 | Spike-αβδ- | 3 | 1–3 | 3 | 6* | 4 | 14 | nd |
2 | 6SG-G12 | TE02 | Spike-αβδο | 14 | 2–7 | 5 | 7 | 6 | 3 | nd |
3 | 7SG-G9 | TE02 | Spike-α--ο | 1 | 4 | 5 | 6 | 6 | 3 | nd |
1 | VHH398 | TE02 | hIgE | 4 | 4–6 | 5 | 1 | 7 | 13 | nd |
2 | VHH428 | TE03 | hIgE | 2 | 3 | 6 | 6 | 7 | 15 | 0.7 |
3 | VHH363 | TE02 | hIgE | 2 | 1 | 5 | 6 | 6 | 11 | 86 |
4 | VHH366 | TE03 | hIgE | 1 | 9 | 5 | 6 | 4 | 12 | 2 |
5 | VHH367 | TE03 | hIgE | 1 | 6 | 6 | 6 | 7 | 15 | 52 |
6 | VHH374 | TE02 | mIgE | 7 | 1–12 | 5 | 8 | 7 | 11 | 9.7 |
7 | VHH375 | TE02 | mIgE | 42 | 3–11 | 5 | 5 | 4 | 6 | 7.8 |
1 | A10 | TE03 | mIgG2c | 4 | 6–11 | 5 | 2 | 4 | 7 | 66 |
2 | C10 | TE03 | mIgG2c | 1 | 9 | 5 | 2 | 7 | 12 | 4 |
1 | A06 | TE02 | hCLEC9a | 1 | 2 | 5 | 1 | 4 | 7 | nd |
2 | A08 | TE03 | hCLEC9a | 3 | 2–3 | 5 | 3 | 4 | 7 | nd |
3 | A17 | TE03 | hCLEC9a | 8 | 1–3 | 5 | 6 | 4 | 8 | nd |
