Fig. 2: Erg6 is essential for A. fumigatus viability. | Nature Communications

Fig. 2: Erg6 is essential for A. fumigatus viability.

From: The sterol C-24 methyltransferase encoding gene, erg6, is essential for viability of Aspergillus species

Fig. 2

A Viability rates of pTetOn-erg6 and pTetOff-erg6 in the indicated doxycycline (DOX) levels using a CFDA staining assay. Hyphae were harvested after GMM culture for 16 h at 30 °C and subsequently stained with 50 μg/ml CFDA for 1 h. Microcolonies that showed bright a green signal were manually enumerated as viable. n = 3 independent experiments. More than 100 microcolonies were measured in each assay. Data is depicted as the mean ± SD. Two-tailed Student t-tests were used for statistical analysis. ns = not significant. B Representative images of pTetOn-erg6 and pTetOff-erg6 microcolonies stained with the live-cell dyes CFDA treated with indicated doxycycline levels. C Conidia of the parental and pTetOff-erg6 strains were initially grown in GMM broth without doxycycline treatment for 8 h to allow the formation of germlings. Subsequently, germling aliquots of 10 µl were transferred to fresh GMM agar plates supplemented with the indicated concentration of doxycycline, and plates were incubated for an additional 48 h.

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