Fig. 7: Downregulation of erg6 does not alter antifungal susceptibility in A. fumigatus. | Nature Communications

Fig. 7: Downregulation of erg6 does not alter antifungal susceptibility in A. fumigatus.

From: The sterol C-24 methyltransferase encoding gene, erg6, is essential for viability of Aspergillus species

Fig. 7

Strip-diffusion MIC assays of the parental and pTetOff-erg6 strains were carried out under the indicated doxycycline (DOX) concentrations. Conidia (2 × 106) suspended in 0.5 ml sterile water were spread evenly over GMM plates and allowed to dry. Commercial test strips embedded with voriconazole, itraconazole, isavuconazole, posaconazole (A) or amphotericin B (B) were applied and plates were incubated for 48 h at 37 °C. The resulting MIC values are indicated at the bottom of each plate image. AMB, amphotericin B, VOR, voriconazole, ITRA, itraconazole, IVU, isavuconazole, POS, posaconazole. (C). The expression levels of abcC and mdr1 under the indicated conditions were analyzed by RT-qPCR. Gene expression was normalized to the reference gene, tubA, and data is presented relative to control group as mean ± SD of log2 fold change. n = 3 independent experiments. Two-tailed Student t-test was used for data analysis.

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