Fig. 8: Repression of erg6 reduces virulence in a murine model of invasive aspergillosis. | Nature Communications

Fig. 8: Repression of erg6 reduces virulence in a murine model of invasive aspergillosis.

From: The sterol C-24 methyltransferase encoding gene, erg6, is essential for viability of Aspergillus species

Fig. 8

A Survival analysis of mice infected with the indicated strains with or without doxycycline (DOX). Mice (n = 10/group) were immune suppressed chemotherapeutically using cyclophosphamide and triamcinolone acetonide as described in Material and Methods and inoculated with 1 × 106 conidia. For the doxycycline treatment arms, doxycycline (100 mg/kg) was supplied by gavage twice a day from Day −3 to Day 3 then once a day from Day 4 to the end of study. Data were analyzed by Mantel-Cox log-rank test comparing doxycycline-treated arms to their no doxycycline control. The pTetOn-erg6 group was compared to the ΔakuB‐pyrG+ group. B–D Representative images of Grocott’s Methenamine Silver-stained lung tissue from mice of A. Lungs (n = 3 / group) were harvested 3 days post-infection. Hyphae are stained black by GMS.

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